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E0151
Sigma
EpiLife™ Keratinocyte Medium
liquid, application tested
This product number has been discontinued but a similar product may be available. Please select the recommended replacement product below (if a direct replacement is available) or use the links to "similar products" to see other possible replacement products.
Description
| Caution | Store at 4°C. Do not freeze. Several components of the medium are light-labile and should not be exposed to light for lengthy periods of time. Protect from light. Prior to use, the medium may be warmed to 37°C. Do not exceed 37°C. When stored at 4°C in the dark, medium which has not been supplemented is stable until the expiration date on the label. Supplemented medium is stable no longer than 1 month at 4°C. |
| Features and Benefits | • Decreases culture time by promoting the expansion of cell numbers • Access to consistent, reproducible cell populations • New primary cells are not acquired as often • Waste is reduced |
| Other Notes | Serum-free medium for the growth of normal human epidermal keratinocytes. Increases the average number of population doublings in vitro of normal low-passage keratinocytes from any source (Figs. 1a, 1b). In comparative testing using standard serum-free keratinocyte media from other manufacturers, product outperformed the competition (Fig. 2). Additionally, normal cell differentiation capabilities are retained. This allows for the expression of differentiation-associated markers (Fig. 3) and the formation of stratified layers in response to the appropriate signals. The basal medium contains HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. Prepared with calcium chloride (0.06mM). Does not contain antibiotics, antimycotics, hormones, growth factors or proteins. |
| To fully support plating and long-term proliferation of human keratinocytes, product must be supplemented with Keratinocyte Medium Supplement (K2007) or Keratinocyte Medium Supplement, BPE-free (K3136). | |
| Legal Information | EpiLife is a trademark of Cascade Biologics, Inc. |
Properties
| sterility | sterile-filtered |
| form | liquid |
| total impurities | Endotoxin, tested |
| suitability | application tested |
| storage temp. | 2-8°C |
Related Products
| No Related Products |
